By Peter Bedson, Helen Parkes, V (Ginny) Saunders, Sue Upton
Introduces the problems of validation and caliber to the bioanalytical neighborhood, in particular addressing DNA-based analyses. Designed to elevate knowledge of the criteria that may impression the validity of DNA research and the construction of extra demanding situations which are linked to the research of actual samples. Spiral.
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Additional info for Analytical Molecular Biology
2 Sample Preparation A sample may require some manipulation in order to maximise the surface area to lysis forces ratio. In some circumstances it may be prudent to surface-clean or sterilise a sample, for example if the target organism is contained within a matrix or higher organism which could have been externally contaminated. Selective separation of the sample could also take place at this stage. 2 In order to achieve this, it may be necessary to homogenise heterogeneous samples in a blender or mortar and pestle before analysis.
Is HMW genomic DNA required or will degraded DNA be tolerated as an analyte? 1. 1 D N A requirements of some common analytical techniques that employ genomic D N A D N A requirements Analytical method Purity Integrity Approx. 1, where DNA was extracted from meat samples. Equal amounts of four types of bovine tissue (A, steak; B, kidney; C stewing steak; D, fat) were extracted using a typical validated method. At the stage prior to DNA precipitation, the extract was divided into four and precipitated as described before an aliquot was electrophoresed on an agarose gel and visualised under UV light.
The property value(s) of the RM should have been established with a precision and an accuracy sufficient to the end use(s) of the RM. Accurate is defined as ‘made by a method having negligible systematic error or bias and by means of measuring instruments or material measures which are traceable to national measurement standards’. 4. Clear documentation concerning the RM and its established property values should be available. Certified reference materials (CRMs) are also sometimes referred to, and are defined as ‘a reference material, one or more of whose property values are certified by a technically valid procedure, accompanied by or traceable to a certificate or other documentation which is issued by a certifying body’.